Mouse heparin proteoglycan. Synthesis by mast cell-fibroblast monolayers during lymphocyte-dependent mast cell proliferation.
نویسندگان
چکیده
From the Laboratory of Clinical Znuestigation, National Institute of Allergy and Infectious Diseases, National Znstitutes of Health, Bethesda, Maryland 20205, Department of Immunology, Faculty of Medicine, Technion-lsrael Institute of Technology, P.O.B. 9649, Haifa, Israel, and Connective Tissue-Aging Research Laboratory, Veterans Administration Outpatient Clinic, Boston, Massachusetts 02108, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 021 I5
منابع مشابه
Fibroblasts induce heparin synthesis in chondroitin sulfate E containing human bone marrow-derived mast cells.
Human bone marrow-derived mast cells (hBMMCs), differentiated in vitro in suspension culture and under the influence of human peripheral blood mononuclear cells conditioned medium (hCM), were tested for their response to recombinant human interleukin-3 (rhIL-3) and for their behavior in different microenvironments. The hBMMCs were incubated in the presence of rhIL-3 and the changes in their pro...
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We investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype. Recombinant rat SCF164 (rrSCF164) induced the development of large numbers of dermal mast cells in normal mice in vivo. Many of these mast cells had features of "connective tissue-type mast cells" (CTMC), in that they were reac...
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Mast cells have been implicated in the pathogenesis of fibrosis because of their increased number in chronic inflammatory reactions. In a previous study, we had shown that human mast cells readily attach and form heterotypic cell-cell contacts when seeded on top of fibroblast monolayers. Here, we report that human mast cells stimulate fibroblast proliferation after cell-cell contact. Proliferat...
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Proteoglycans synthesized in cultured mast cells derived from horse serum-immunized lymph node cells were analyzed. Treatment of the 35S-proteoglycans extracted from these cells with either chondroitinase ABC or AC resulted in 95% +/- 7% and 84% +/- 7%, respectively (mean +/- S.E., n = 3), of the radioactivity associated with disaccharides eluting in the included volume of PD-10. The 35S-proteo...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 257 15 شماره
صفحات -
تاریخ انتشار 1982